Yurchenko V, O'Connor M, Dai W W, Guo H, Toole B, Sherry B, Bukrinsky M
The Picower Institute for Medical Research, Manhasset, New York 11030, USA.
Biochem Biophys Res Commun. 2001 Nov 9;288(4):786-8. doi: 10.1006/bbrc.2001.5847.
Cyclophilins A and B (CyPA and CyPB) are cyclosporin A binding proteins that can be secreted in response to inflammatory stimuli. We recently identified CD147 as a cell-surface receptor for CyPA and demonstrated that CD147 is an essential component in the CyPA-initiated signaling cascade that culminates in ERK activation and chemotaxis. Here we demonstrate that CD147 also serves as a receptor for CyPB. CyPB induced Ca(2+) flux and chemotaxis of CD147-transfected, but not control, CHO cells, and the chemotactic response of primary human neutrophils to CyPB was blocked by antibodies to CD147. These results suggest that CD147 serves as a receptor for extracellular cyclophilins.
亲环蛋白A和B（CyPA和CyPB）是可响应炎症刺激而分泌的环孢菌素A结合蛋白。我们最近鉴定出CD147是CyPA的细胞表面受体，并证明CD147是CyPA启动的信号级联反应中的重要组成部分，该信号级联反应最终导致ERK激活和趋化作用。在此我们证明CD147也是CyPB的受体。CyPB诱导了转染CD147的CHO细胞（而非对照CHO细胞）的Ca(2+)内流和趋化作用，并且抗CD147抗体阻断了原代人中性粒细胞对CyPB的趋化反应。这些结果表明CD147是细胞外环孢菌素的受体。
