Fukui T, Nonomura N, Tokizane T, Sato E, Ono Y, Harada Y, Nishimura K, Takahara S, Okuyama A
Department of Specific Organ Regulation, Urology, Osaka University Graduate School of Medicine, Osaka, Japan.
Mol Urol. 2001 Spring;5(1):19-23. doi: 10.1089/109153601750124249.
We examined the expression of mRNA of human telomerase reverse transcriptase (hTERT), a catalytic subunit of the telomerase complex, in bladder washings as a tumor marker for the detection of bladder cancer.
Bladder washings were obtained from 63 patients. We examined the expression of hTERT mRNA by the nested reverse transcription-polymerase chain reaction (RT-PCR) method and also measured the relative expressions of hTERT mRNA and beta(2)-microglobulin (beta(2)-MG) in these samples by RT-PCR analysis. Correlations between the relative expression of hTERT mRNA and clinical features were analyzed. We also compared the sensitivity of this assay with that of urinary cytology.
By nested RT-PCR, we detected three false-positive cases (11%) in the control group. Therefore, the relative expression values of hTERT mRNA and beta(2)-MG correlated strongly with tumor size, but not with multiplicity or histologic grade. When the cut-off value of the expression was fixed at 0.27%, the sensitivity and specificity of this assay were 74% and 93%, respectively. This assay was more sensitive than urinary cytology for the detection of bladder cancer.
These results suggest that the relative expression of hTERT mRNA in bladder washings is useful in screening for bladder cancer. Relative expression is of assistance in diagnosing bladder cancer.
我们检测了端粒酶复合物催化亚基人端粒酶逆转录酶（hTERT）的mRNA在膀胱冲洗液中的表达，将其作为检测膀胱癌的肿瘤标志物。
收集63例患者的膀胱冲洗液。我们采用巢式逆转录-聚合酶链反应（RT-PCR）法检测hTERT mRNA的表达，并通过RT-PCR分析测定这些样本中hTERT mRNA和β2-微球蛋白（β2-MG）的相对表达量。分析hTERT mRNA相对表达量与临床特征之间的相关性。我们还将该检测方法的灵敏度与尿细胞学检查的灵敏度进行了比较。
通过巢式RT-PCR，我们在对照组中检测到3例假阳性病例（11%）。因此，hTERT mRNA和β2-MG的相对表达值与肿瘤大小密切相关，但与肿瘤数量或组织学分级无关。当表达的截断值固定为0.27%时，该检测方法的灵敏度和特异度分别为74%和93%。该检测方法在检测膀胱癌方面比尿细胞学检查更敏感。
这些结果表明，膀胱冲洗液中hTERT mRNA的相对表达量有助于膀胱癌的筛查。相对表达量有助于膀胱癌的诊断。
