Mornet D, Bonet-Kerrache A
Muscles et Pathologies, INSERM U128, IFR 24, Institut Bouisson-Bertrand, 778, Rue de la Croix Verte, 34196 Cedex 5, Montpellier, France.
Biochim Biophys Acta. 2001 Oct 18;1549(2):197-203. doi: 10.1016/s0167-4838(01)00260-6.
Neurocalcin is an N-myristoylated calcium-binding protein which belongs to a novel family of neuronal calcium sensors. Here we show, by cosedimentation, co-immunoprecipitation and cross-linking approaches, that myristoylated neurocalcin directly interacts with actin in a calcium-dependent manner. We used EDC cross-linking and obtained one novel 64 kDa entity composed of one actin molecule and one neurocalcin molecule, as demonstrated with IAEDANS-actin and neurocalcin-specific antibodies. This interaction could modulate the rod outer segment-guanylate cyclase 1-neurocalcin interface.
神经钙蛋白是一种N-肉豆蔻酰化的钙结合蛋白，属于一类新型的神经元钙传感器家族。在这里，我们通过共沉降、共免疫沉淀和交联方法表明，肉豆蔻酰化的神经钙蛋白以钙依赖的方式直接与肌动蛋白相互作用。我们使用EDC交联，得到了一个由一个肌动蛋白分子和一个神经钙蛋白分子组成的新的64 kDa实体，这通过IAEDANS-肌动蛋白和神经钙蛋白特异性抗体得以证明。这种相互作用可能会调节视杆外段-鸟苷酸环化酶1-神经钙蛋白界面。
