Burton S C, Harding D R
Biochemical Recovery Group, Centre for Bioprocess Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
J Biochem Biophys Methods. 2001 Oct 30;49(1-3):275-87. doi: 10.1016/s0165-022x(01)00204-4.
The role of chromatography in capture is reviewed in terms of the special requirements imposed by the processing of very crude feedstocks. Adsorption methods which are not significantly affected by variations of feedstock ionic strength are highlighted. Methods are compared in terms of simplicity, robustness, selectivity and ease of elution. The application of such methods to enzyme and antibody purifications is summarised. Particular emphasis is placed on high ligand density methods, which have potential for broad-spectrum application.
本文从极粗原料加工所带来的特殊要求方面，对色谱法在捕获过程中的作用进行了综述。重点介绍了不受原料离子强度变化显著影响的吸附方法。并从简单性、稳健性、选择性和洗脱难易程度等方面对各种方法进行了比较。总结了这些方法在酶和抗体纯化中的应用。特别强调了高配体密度方法，该方法具有广泛应用的潜力。
