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β-半乳糖苷酶基因上游的多聚嘌呤-嘧啶序列影响酿酒酵母中的基因表达。

Poly purine.pyrimidine sequences upstream of the beta-galactosidase gene affect gene expression in Saccharomyces cerevisiae.

作者信息

Maiti A K, Brahmachari S K

机构信息

Molecular Biophysics Unit, Indian Institute of Science, Bangalore-560012, India.

出版信息

BMC Mol Biol. 2001;2:11. doi: 10.1186/1471-2199-2-11. Epub 2001 Oct 8.

DOI:10.1186/1471-2199-2-11
PMID:11696239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC59624/
Abstract

BACKGROUND

Poly purine.pyrimidine sequences have the potential to adopt intramolecular triplex structures and are overrepresented upstream of genes in eukaryotes. These sequences may regulate gene expression by modulating the interaction of transcription factors with DNA sequences upstream of genes.

RESULTS

A poly purine.pyrimidine sequence with the potential to adopt an intramolecular triplex DNA structure was designed. The sequence was inserted within a nucleosome positioned upstream of the beta-galactosidase gene in yeast, Saccharomyces cerevisiae, between the cycl promoter and gal 10 Upstream Activating Sequences (UASg). Upon derepression with galactose, beta-galactosidase gene expression is reduced 12-fold in cells carrying single copy poly purine.pyrimidine sequences. This reduction in expression is correlated with reduced transcription. Furthermore, we show that plasmids carrying a poly purine.pyrimidine sequence are not specifically lost from yeast cells.

CONCLUSION

We propose that a poly purine.pyrimidine sequence upstream of a gene affects transcription. Plasmids carrying this sequence are not specifically lost from cells and thus no additional effort is needed for the replication of these sequences in eukaryotic cells.

摘要

背景

多聚嘌呤-嘧啶序列有形成分子内三链结构的潜力，并且在真核生物基因上游过度富集。这些序列可能通过调节转录因子与基因上游DNA序列的相互作用来调控基因表达。

结果

设计了一个有潜力形成分子内三链DNA结构的多聚嘌呤-嘧啶序列。该序列被插入酿酒酵母中位于β-半乳糖苷酶基因上游的一个核小体内部，处于环启动子和gal 10上游激活序列（UASg）之间。在用半乳糖解除抑制后，携带单拷贝多聚嘌呤-嘧啶序列的细胞中β-半乳糖苷酶基因表达降低了12倍。这种表达的降低与转录减少相关。此外，我们表明携带多聚嘌呤-嘧啶序列的质粒不会从酵母细胞中特异性丢失。

结论

我们提出基因上游的多聚嘌呤-嘧啶序列会影响转录。携带该序列的质粒不会从细胞中特异性丢失，因此在真核细胞中复制这些序列无需额外的努力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/a4f9a4bd2a01/1471-2199-2-11-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/a4e41acbb809/1471-2199-2-11-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/2b4e4ace3cd2/1471-2199-2-11-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/50a75317c285/1471-2199-2-11-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/a48b5fab76d8/1471-2199-2-11-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/a4f9a4bd2a01/1471-2199-2-11-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/a4e41acbb809/1471-2199-2-11-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/2b4e4ace3cd2/1471-2199-2-11-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/50a75317c285/1471-2199-2-11-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/a48b5fab76d8/1471-2199-2-11-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/473b/59624/a4f9a4bd2a01/1471-2199-2-11-5.jpg

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