Morlon-Guyot J, Mucciolo-Roux F, Rodriguez Sanoja R, Guyot J P
Laboratoire de Biotechnologie Microbienne Tropicale (LBMT), Institut de Recherche pour le Développement (IRD, ex ORSTOM), 34032 Montpellier, France.
DNA Seq. 2001 Jul;12(1):27-37. doi: 10.3109/10425170109042048.
Primers and probes were established from the sequences of the alpha-amylase genes (amyA) of L. amylovorus CIP 102989 and of L. plantarum A6 (Giraud and Cuny 1997). They were successfully used for the detection of the amyA gene in L. manihotivorans strain LMG 18010T and a 2842 bp region, containing the entire gene (2706 bp) with its putative promoter has been sequenced. More than 98% nucleotide sequence identities was found with L. amylovorus and L. plantarum amyA genes. The deduced amino acid sequence shares more than 96% amino acid sequence identities with L. amylovorus and L. plantarum alpha-amylases, and also 65% and 59% identities with the alpha-amylases of B. subtilis and S. bovis, respectively. The 3' terminal part of L. manihotivorans LMG 18010T amyA gene contained four repeated sequences (SRU). The amyA genes of the three lactobacilli species differed mainly in the number of SRU and in the size of the flanking regions of the SRU.
引物和探针是根据嗜酸乳杆菌CIP 102989和植物乳杆菌A6的α-淀粉酶基因（amyA）序列设计的（吉罗和库尼，1997年）。它们成功用于检测木薯嗜酸乳杆菌菌株LMG 18010T中的amyA基因，并且已对一个2842 bp的区域进行了测序，该区域包含整个基因（2706 bp）及其推定的启动子。发现该基因与嗜酸乳杆菌和植物乳杆菌的amyA基因的核苷酸序列同一性超过98%。推导的氨基酸序列与嗜酸乳杆菌和植物乳杆菌的α-淀粉酶的氨基酸序列同一性超过96%，与枯草芽孢杆菌和牛链球菌的α-淀粉酶的同一性分别为65%和59%。木薯嗜酸乳杆菌LMG 18010T amyA基因的3'末端部分包含四个重复序列（SRU）。这三种乳杆菌的amyA基因主要在SRU的数量和SRU侧翼区域的大小上有所不同。
