Alarcón-Chaidez F J, Bender C L
127 Noble Research Center, Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA.
DNA Seq. 2001 Jul;12(1):77-84. doi: 10.3109/10425170109042054.
sigma 54, which is encoded by rpoN, is required for a variety of metabolic functions in bacteria including the utilization of alternative carbon and nitrogen sources, nitrogen fixation, and the expression of virulence determinants. Sequence analysis of a 3,020-bp DNA fragment from the plant pathogen Pseudomonas syringae pv. glycinea PG4180 revealed four ORFs designated rpoN, orfA, orfB, and orfC delta, which were related to rpoN and rpoN-associated genes from other microorganisms. The rpoN upstream region in P. syringae contained two overlapping promoters, which may suggest a complex regulatory pattern. This is the first study describing the organization of the rpoN locus in P. syringae.
由rpoN编码的σ54是细菌多种代谢功能所必需的，这些功能包括利用替代碳源和氮源、固氮以及毒力决定因素的表达。对植物病原菌丁香假单胞菌大豆致病变种PG4180的一个3020 bp DNA片段进行序列分析，发现了四个开放阅读框，分别命名为rpoN、orfA、orfB和orfC delta，它们与其他微生物的rpoN及rpoN相关基因有关。丁香假单胞菌的rpoN上游区域包含两个重叠启动子，这可能暗示着一种复杂的调控模式。这是首次描述丁香假单胞菌中rpoN基因座组织的研究。
