Kotani A, Ishikawa T, Matsumura Y, Ichinohe T, Ohno H, Hori T, Uchiyama T
Department of Hematology/Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Blood. 2001 Nov 15;98(10):3162-4. doi: 10.1182/blood.v98.10.3162.
There is no reliable laboratory indicator of the onset of chronic graft-versus-host disease (cGVHD). This study looks at whether the expression of OX40, a member of the tumor necrosis factor receptor family, is related to the development of cGVHD in patients who underwent allogeneic hematopoietic stem cell transplantation. Peripheral blood mononuclear cells from 22 patients after day 100 were subjected to multicolor flow cytometry. The percentages of both OX40+CD4+ and OX40+CD8+ T cells were significantly higher in patients with cGVHD than those without (P <.0001 and P =.001, respectively). Serial analyses showed that OX40+CD4+ T cells elevated before the onset of cGVHD and closely correlated with the therapeutic response. The expression of CD25, CD69, and HLA-DR was partially detectable on OX40+ T cells. These results indicate that serial measurement of OX40+ T cells is useful for predicting the onset as well as the therapeutic response of cGVHD and raise a possibility that the OX40/gp34 system is involved in the pathogenesis of cGVHD.
目前尚无可靠的实验室指标来指示慢性移植物抗宿主病（cGVHD）的发病。本研究旨在探讨肿瘤坏死因子受体家族成员OX40的表达是否与接受异基因造血干细胞移植患者的cGVHD发生有关。对22例移植后100天以上患者的外周血单个核细胞进行多色流式细胞术检测。cGVHD患者的OX40+CD4+和OX40+CD8+T细胞百分比均显著高于无cGVHD患者（分别为P <.0001和P =.001）。系列分析表明，OX40+CD4+T细胞在cGVHD发病前升高，且与治疗反应密切相关。OX40+T细胞上可部分检测到CD25、CD69和HLA-DR的表达。这些结果表明，连续检测OX40+T细胞有助于预测cGVHD的发病及治疗反应，并提示OX40/gp34系统可能参与了cGVHD的发病机制。
