黄色粘球菌发育过程中mrp基因的分析。
Analyses of mrp genes during Myxococcus xanthus development.
作者信息
Sun H, Shi W
机构信息
Molecular Biology Institute and School of Dentistry, University of California, 10833 Le Conte Avenue, Los Angeles, CA 90095-1668, USA.
出版信息
J Bacteriol. 2001 Dec;183(23):6733-9. doi: 10.1128/JB.183.23.6733-6739.2001.
Abstract
Myxococcus xanthus is a gram-negative soil bacterium that undergoes development under starvation conditions. Our previous study identified a new genetic locus, mrp, which is required for both fruiting body formation and sporulation. The locus encodes two transcripts: mrpAB, which consists of a histidine kinase and an NtrC-like response regulator, and mrpC, a cyclic AMP receptor protein family transcription activator. In this study, we used genetic and biochemical analyses to investigate the possible interactions between the mrp genes and other known developmental genes and events. These studies show that the mrp genes possibly function after A-signaling and (p)ppGpp but before C-signaling and that they regulate various early and late developmental genes and events.
摘要
黄色黏球菌是一种革兰氏阴性土壤细菌，在饥饿条件下会经历发育过程。我们之前的研究鉴定出一个新的基因座mrp，它是子实体形成和孢子形成所必需的。该基因座编码两种转录本：mrpAB，由一个组氨酸激酶和一个NtrC样应答调节因子组成；以及mrpC，一种环腺苷酸受体蛋白家族转录激活因子。在本研究中，我们使用遗传学和生物化学分析来研究mrp基因与其他已知发育基因及事件之间可能的相互作用。这些研究表明，mrp基因可能在A信号和（p）ppGpp之后但在C信号之前发挥作用，并且它们调节各种早期和晚期发育基因及事件。
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本文引用的文献
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Genetic studies of mrp, a locus essential for cellular aggregation and sporulation of Myxococcus xanthus.对mrp的遗传学研究，mrp是黄色粘球菌细胞聚集和孢子形成所必需的一个基因座。
J Bacteriol. 2001 Aug;183(16):4786-95. doi: 10.1128/JB.183.16.4786-4795.2001.
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C-signal: a cell surface-associated morphogen that induces and co-ordinates multicellular fruiting body morphogenesis and sporulation in Myxococcus xanthus.C信号：一种与细胞表面相关的形态发生素，可诱导并协调黄色粘球菌中多细胞子实体的形态发生和孢子形成。
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The guanosine nucleotide (p)ppGpp initiates development and A-factor production in myxococcus xanthus.鸟苷核苷酸（p）ppGpp启动黄色黏球菌的发育和A因子产生。
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C factor, a cell-surface-associated intercellular signaling protein, stimulates the cytoplasmic Frz signal transduction system in Myxococcus xanthus.C因子是一种与细胞表面相关的细胞间信号蛋白，可刺激黄色粘球菌中的细胞质Frz信号转导系统。
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Chemotaxis plays a role in the social behaviour of Myxococcus xanthus.趋化作用在黄色粘球菌的社会行为中发挥作用。
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