Clifford G M, Kraemer F B, Yeaman S J, Vernon R G
Hannah Research Institute, Ayr, UK.
Metabolism. 2001 Nov;50(11):1264-9. doi: 10.1053/meta.2001.27216.
The removal of the litter from lactating rats results in a decrease in the lipolytic response to catecholamines in maternal adipocytes; this effect can be prevented by concomitant treatment of the rats with growth hormone. The decrease in response to catecholamines following litter removal was not due to a change in the amount of either hormone-sensitive lipase (HSL) or perilipin per adipocyte or in the proportion of either of these proteins associated with the fat droplet. Incubation in vitro with isoproterenol did not cause any apparent net translocation of HSL to the fat droplet in adipocytes from the mature female rats in any state used in this study, but isoproterenol did cause a movement of perlipin away from the fat droplet. This translocation of perilipin was not altered by litter removal. Thus, the decrease in response to catecholamines found on litter removal from lactating rats appears to be due to a diminished ability to activate HSL associated with fat droplet.
从哺乳期大鼠中移除幼崽会导致母体脂肪细胞对儿茶酚胺的脂解反应降低；用生长激素同时处理大鼠可预防这种效应。移除幼崽后对儿茶酚胺反应的降低并非由于每个脂肪细胞中激素敏感性脂肪酶（HSL）或围脂滴蛋白的量发生变化，也不是由于这些蛋白质与脂滴相关的比例发生变化。在本研究使用的任何状态下，成熟雌性大鼠的脂肪细胞与异丙肾上腺素进行体外孵育，均未导致HSL向脂滴发生任何明显的净转运，但异丙肾上腺素确实导致围脂滴蛋白从脂滴上移开。这种围脂滴蛋白的转运不受移除幼崽的影响。因此，从哺乳期大鼠中移除幼崽后发现的对儿茶酚胺反应的降低似乎是由于激活与脂滴相关的HSL的能力减弱所致。
