Dunphy C H, Polski J M, Johns G, Evans H L, Gardner L J
Division of Hematopathology, Department of Pathology, St. Louis University Health Sciences Center, St. Louis, Missouri, MO 63014, USA.
Leuk Lymphoma. 2001 Jun;42(1-2):215-9. doi: 10.3109/10428190109097693.
A diagnosis of the hypogranular variant of acute promyelocytic leukemia (APLv) may be difficult to establish based on cytomorphology alone. However, the great majority of cases have a classical immunophenotype by flow cytometric immunophenotyping (FCI) (CD13+, CD33+, dim CD64+, HLA-DR-, and CD34-) and a classical enzyme cytochemical (EC) staining pattern. [intensely staining with myeloperoxidase, Sudan Black B, and chloroacetate esterase (CAE) and negative with alpha'-naphthyl acetate and butyrate esterases]. Although the immunophenotype of APLv by FCI has varied in the literature (HLA-DR +/- and CD34 +/-), the EC staining pattern has remained constant. We report a case of APLv with characteristic cytomorphology, compatible FCI data (CD13+, CD33+, dim CD64+, HLA-DR +/-, and CD34-), chromosomal detection of t(15; 17), and molecular detection of the PML/RAR alpha fusion gene; however, staining of the leukemic cells with CAE was quite uncharacteristic. We describe our findings.
仅基于细胞形态学可能难以确诊急性早幼粒细胞白血病低颗粒变异型（APLv）。然而，绝大多数病例通过流式细胞术免疫表型分析（FCI）具有经典免疫表型（CD13 +、CD33 +、CD64弱阳性、HLA - DR -、CD34 -）以及经典酶细胞化学（EC）染色模式[髓过氧化物酶、苏丹黑B和氯乙酸酯酶（CAE）强阳性染色，α - 萘乙酸酯酶和丁酸酯酶阴性]。尽管文献中FCI检测的APLv免疫表型有所不同（HLA - DR +/-和CD34 +/-），但EC染色模式保持不变。我们报告1例具有特征性细胞形态学、符合FCI数据（CD13 +、CD33 +、CD64弱阳性、HLA - DR +/-、CD34 -）、t(15; 17)染色体检测结果及PML/RARα融合基因分子检测结果的APLv病例；然而，白血病细胞的CAE染色极不典型。我们描述了我们的发现。
