东亚钳蝎两种蝎毒肽BmTXKbeta和BmTXKS2的基因组结构
Genomic organization of BmTXKbeta and BmTXKS2, two scorpion venom peptides from Buthus martensii Karsch.
作者信息
Cao Z, Zhu S, Li W, Jiang D, He X, Liu H
机构信息
Department of Biotechnology, College of Life Sciences, Wuhan University, China.
出版信息
IUBMB Life. 2001 May;51(5):305-8. doi: 10.1080/152165401317190815.
To elucidate genomic organization of BmTXKbeta and BmTXKS2, two scorpion venom peptides from Chinese scorpion Buthus martensii Karsch(BmK) were first isolated and their genomic regions characterized using the PCR method. Analysis of nucleotide sequence shows that there exists different intron location in the venom genes. The region encoding mature peptide of BmTXKbeta is disrupted by an intron with 886 bp, whereas the intron of BmTXKS2 is located within its propeptide coding region, which is different from other scorpion toxin genes with their introns within the signal peptide coding region.
为阐明来自中国蝎子东亚钳蝎（BmK）的两种蝎毒肽BmTXKbeta和BmTXKS2的基因组结构，首先分离了这两种肽，并使用PCR方法对其基因组区域进行了表征。核苷酸序列分析表明，毒液基因中存在不同的内含子位置。编码BmTXKbeta成熟肽的区域被一个886 bp的内含子打断，而BmTXKS2的内含子位于其前肽编码区域内，这与其他内含子位于信号肽编码区域内的蝎毒素基因不同。
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