Pieroni L, La Monica N
Cell Biology and Biophysics Department, Heidelberg, Germany.
Curr Opin Mol Ther. 2001 Oct;3(5):464-7.
The use of baculovirus vectors for gene expression in mammalian cells is in continuous expansion. These vectors do not replicate in mammalian cells, do not cause a cytopathic effect upon infection and are able to carry large DNA inserts. Baculovirus vectors have been shown to transduce various cell types in vitro and in vivo with significant efficiency leading to stable gene expression. This review focuses on recent developments with baculovirus that highlight its potential usefor new gene therapy strategies.
杆状病毒载体在哺乳动物细胞中的基因表达应用正在不断扩展。这些载体在哺乳动物细胞中不复制，感染时不会引起细胞病变效应，并且能够携带大片段DNA插入物。杆状病毒载体已被证明能在体外和体内高效转导多种细胞类型，从而实现稳定的基因表达。本文综述聚焦于杆状病毒的最新进展，这些进展凸显了其在新基因治疗策略中的潜在用途。
