Guzman N A, Stubbs R J
Bioanalytical Drug Metabolism, The R. W. Johnson Pharmaceutical Research Institute, Raritan, NJ 08869, USA.
Electrophoresis. 2001 Oct;22(17):3602-28. doi: 10.1002/1522-2683(200109)22:17<3602::AID-ELPS3602>3.0.CO;2-X.
Much attention has recently been directed to the development and application of online sample preconcentration and microreactions in capillary electrophoresis using selective adsorbents based on chemical or biological specificity. The basic principle involves two interacting chemical or biological systems with high selectivity and affinity for each other. These molecular interactions in nature usually involve noncovalent and reversible chemical processes. Properly bound to a solid support, an "affinity ligand" can selectively adsorb a "target analyte" found in a simple or complex mixture at a wide range of concentrations. As a result, the isolated analyte is enriched and highly purified. When this affinity technique, allowing noncovalent chemical interactions and biochemical reactions to occur, is coupled on-line to high-resolution capillary electrophoresis and mass spectrometry, a powerful tool of chemical and biological information is created. This paper describes the concept of biological recognition and affinity interaction on-line with high-resolution separation, the fabrication of an "analyte concentrator-microreactor", optimization conditions of adsorption and desorption, the coupling to mass spectrometry, and various applications of clinical and pharmaceutical interest.
最近，人们将大量注意力投向了基于化学或生物特异性的选择性吸附剂在毛细管电泳中在线样品预浓缩和微反应的开发与应用。其基本原理涉及两个相互作用的化学或生物系统，它们彼此之间具有高选择性和亲和力。自然界中的这些分子相互作用通常涉及非共价和可逆的化学过程。“亲和配体”与固体支持物恰当结合后，能够在很宽的浓度范围内选择性吸附简单或复杂混合物中的“目标分析物”。结果，被分离的分析物得以富集并高度纯化。当这种允许非共价化学相互作用和生化反应发生的亲和技术与高分辨率毛细管电泳和质谱在线联用，就创造出了一种强大的化学和生物信息工具。本文描述了生物识别和亲和相互作用与高分辨率分离在线联用的概念、“分析物浓缩器 - 微反应器”的制备、吸附和解吸的优化条件、与质谱的联用以及临床和药学领域的各种应用。
