用于DNA突变检测的空间温度梯度毛细管电泳

Spatial temperature gradient capillary electrophoresis for DNA mutation detection.

作者信息

Zhu L, Lee H K, Lin B, Yeung E S

机构信息

Department of Chemistry National University of Singapore, Republic of Singapore.

出版信息

Electrophoresis. 2001 Oct;22(17):3683-7. doi: 10.1002/1522-2683(200109)22:17<3683::AID-ELPS3683>3.0.CO;2-B.

DOI:10.1002/1522-2683(200109)22:17<3683::AID-ELPS3683>3.0.CO;2-B

PMID:11699906

Abstract

A continuous spatial temperature gradient was established in capillary electrophoresis by using a simple temperature control device. The temperature profile along the capillary was predicted by theoretical calculations. A nearly linear spatial temperature gradient was established and applied to DNA mutation detection. By spanning a wide temperature range, it was possible to perform simultaneous heteroduplex analysis for various mutation types that have different melting temperatures.

摘要

通过使用一种简单的温度控制装置，在毛细管电泳中建立了连续的空间温度梯度。通过理论计算预测了沿毛细管的温度分布。建立了近乎线性的空间温度梯度并将其应用于DNA突变检测。通过跨越较宽的温度范围，可以对具有不同解链温度的各种突变类型进行同步异源双链分析。