Wen E, Rathore A S, Horváth C
Department of Chemical Engineering, Yale University, New Haven, CT 06520-8286, USA.
Electrophoresis. 2001 Oct;22(17):3720-7. doi: 10.1002/1522-2683(200109)22:17<3720::AID-ELPS3720>3.0.CO;2-Z.
A major impediment to enhancing the speed of separation in capillary electrochromatography (CEC) is the upper limit on the electroosmotic flow (EOF) velocity by the maximal zeta potential of the chromatographic surface. Here, a new approach to speeding up EOF, suggested by Yang and El Rassi (Electrophoresis 1999, 20,18-23), is examined critically. It entails the use of a tandem arrangement of a separating column and an auxiliary column, the sole function of which is to boost EOF velocity in the separating column and thus facilitate faster analysis by CEC. Based on the principle of conservation of mass and current and using experimental data obtained in a wide range of conditions, the flow velocities in the separating and auxiliary columns were evaluated. The results show that an equidiameter open tubular auxiliary column offers a greater enhancement of EOF velocity than a packed column. Nevertheless, within the scope of the experiments the enhancement of EOF velocity by as much as 50% by using open tubular auxiliary columns has been obtained.
在毛细管电色谱（CEC）中，提高分离速度的一个主要障碍是色谱表面的最大zeta电位对电渗流（EOF）速度的上限。在此，对Yang和El Rassi（《电泳》，1999年，20卷，18 - 23页）提出的一种加快EOF的新方法进行了严格研究。该方法需要使用一个分离柱和一个辅助柱的串联装置，辅助柱的唯一功能是提高分离柱中的EOF速度，从而便于通过CEC进行更快的分析。基于质量和电流守恒原理，并利用在广泛条件下获得的实验数据，对分离柱和辅助柱中的流速进行了评估。结果表明，等直径开管辅助柱比填充柱能更大程度地提高EOF速度。然而，在实验范围内，使用开管辅助柱已实现将EOF速度提高多达50%。
