Sanders G M, Hewish M J, Coulson B S
Department of Microbiology and Immunology, The University of Melbourne, Victoria, Australia.
Arch Virol. 2001;146(9):1831-40. doi: 10.1007/s007050170069.
The role of integrins previously implicated as rotavirus receptors in determining cellular susceptibility to SA11 rotavirus was studied, using phorbol dibutyrate (PDB) treatment of K562 cells to induce megakaryocytic differentiation. Expression of alpha2beta1 integrin was detected after 2 days in PDB, and peaked after PDB treatment for 4-7 days. SA11 titres were increased by 1.8- to 10.8-fold over untreated cells after PDB treatment for 2-7 days, and correlated with levels of alpha2beta1 integrin expression in PDB-treated K562 cells.
利用佛波醇二丁酸酯（PDB）处理K562细胞以诱导巨核细胞分化，研究了先前被认为是轮状病毒受体的整合素在决定细胞对SA11轮状病毒易感性方面的作用。在PDB处理2天后检测到α2β1整合素的表达，并在PDB处理4 - 7天后达到峰值。PDB处理2 - 7天后，SA11滴度比未处理的细胞增加了1.8至10.8倍，并且与PDB处理的K562细胞中α2β1整合素的表达水平相关。
