King A J, Berbers G, van Oirschot H F, Hoogerhout P, Knipping K, Mooi F R
Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands.
Microbiology (Reading). 2001 Nov;147(Pt 11):2885-95. doi: 10.1099/00221287-147-11-2885.
In several countries pertussis is re-emerging, despite a high vaccination coverage. It is suggested that antigenic divergence between Bordetella pertussis vaccine strains and circulating strains, in particular with respect to pertactin, has contributed to pertussis re-emergence. Polymorphism in pertactin is essentially limited to region 1, which is composed of repeats and is located adjacent to an Arg-Gly-Asp motif implicated in adherence. Evidence is provided for the immunological relevance of polymorphism in region 1. Region 1 was found to contain a B-cell epitope recognized in both humans and mice. Furthermore, variation in region 1 affected antibody binding and, in a mouse respiratory infection model, the efficacy of a whole-cell vaccine. Moreover, passive and active immunization indicated that region 1 confers protective immunity. An mAb directed against a linear conserved epitope conferred cross-immunity against isolates with distinct pertactin variants. The results indicate an important role of region 1 of pertactin in immunity.
在几个国家，尽管疫苗接种覆盖率很高，但百日咳仍在重新出现。有人认为，百日咳博德特氏菌疫苗株与流行株之间的抗原差异，特别是在百日咳杆菌黏附素方面的差异，促成了百日咳的重新出现。百日咳杆菌黏附素的多态性基本上局限于区域1，该区域由重复序列组成，位于与黏附有关的精氨酸 - 甘氨酸 - 天冬氨酸基序相邻的位置。有证据表明区域1多态性具有免疫相关性。发现区域1包含在人和小鼠中都能识别的B细胞表位。此外，区域1的变异影响抗体结合，并且在小鼠呼吸道感染模型中，影响全细胞疫苗的效力。而且，被动和主动免疫表明区域1赋予保护性免疫。一种针对线性保守表位的单克隆抗体赋予了针对具有不同百日咳杆菌黏附素变体的分离株的交叉免疫。结果表明百日咳杆菌黏附素区域1在免疫中起重要作用。
