Roth C M, Yarmush M L
Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School and Shriners Burns Hospital, Boston, Massachusetts 02114, USA.
Annu Rev Biomed Eng. 1999;1:265-97. doi: 10.1146/annurev.bioeng.1.1.265.
Driven by advances in the acquisition of genetic sequence information and the ability to manipulate small quantities of nucleic acid, a number of technologies are emerging that exploit nucleic acids for research, diagnostic, and therapeutic utility. In this review, we cover three technologies based on nucleic acids--DNA microarrays, antisense technology, and gene therapy--that are especially promising and may make a substantial impact in the laboratory and in the clinic during the coming years. For each of these areas, an overview of the current status and applications is provided, followed by a discussion of critical issues and challenges to be faced for further advancement of the technology; an emphasis is placed on quantitative and engineering aspects.
在获取基因序列信息以及操纵少量核酸能力的进步推动下，涌现出了许多利用核酸用于研究、诊断和治疗用途的技术。在本综述中，我们涵盖了基于核酸的三种技术——DNA微阵列、反义技术和基因治疗——这些技术特别有前景，并且在未来几年可能会在实验室和临床中产生重大影响。对于这些领域中的每一个，我们都提供了当前状态和应用的概述，随后讨论了技术进一步发展所面临的关键问题和挑战；重点放在定量和工程方面。
