血清对乳腺发育过程中体外乳腺上皮细胞增殖的影响。
Effects of serum on mammary epithelial proliferation in vitro during mammary gland development.
作者信息
Hsueh H W, Stockdale F E
出版信息
J Cell Biol. 1975 Aug;66(2):243-50. doi: 10.1083/jcb.66.2.243.
The proliferative response of mammary gland epithelium from nonpregnant, pregnant, and lactating mice to mammary serum factor and insulin was studied in vitro. Mammary gland epiithelium from nonpregnant and lactating animals has a delayed proliferative response to mammary serum factor and insulin when compared to the response of epithelium from pregnant animals. The results show that as the animals go through pregnancy into lactation the mammary gland epithelium becomes less responsive to mammary serum factor while it retains its responsiveness to insulin. The concentration of mammary serum factor in sera from animals at various physiological stages is constant. Sera from hypophysectomized rats, on the other hand, show a 50% drop in mammary serum factor activity. This loss of activity cannot be reversed by injecting prolactin, 17-beta-estradiol, or growth hormone into the hypophysectomized animals. A hypothesis that the mammary gland is composed of two proliferative epithelial populations is developed, and the possible role of prolactin in stimulating DNA synthesis is discussed.
研究了非妊娠、妊娠和泌乳小鼠乳腺上皮细胞对乳腺血清因子和胰岛素的增殖反应。与妊娠动物的上皮细胞反应相比，非妊娠和泌乳动物的乳腺上皮细胞对乳腺血清因子和胰岛素的增殖反应延迟。结果表明，随着动物从妊娠进入泌乳期，乳腺上皮细胞对乳腺血清因子的反应性降低，而对胰岛素仍保持反应性。处于不同生理阶段动物血清中乳腺血清因子的浓度是恒定的。另一方面，垂体切除大鼠的血清中乳腺血清因子活性下降了50%。向垂体切除的动物注射催乳素、17-β-雌二醇或生长激素并不能恢复这种活性丧失。由此提出了一个假说，即乳腺由两个增殖性上皮细胞群体组成，并讨论了催乳素在刺激DNA合成中的可能作用。
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