Kataoka K, Suzaki E, Komura K
Department of Anatomy, Hiroshima University School of Medicine, 2-3 Kasumi-1-chome, Minami-ku, Hiroshima 734-8551, Japan.
Histochem Cell Biol. 2001 Oct;116(4):329-35. doi: 10.1007/s004180100322.
The three-dimensional structure of the Golgi apparatus was studied in goblet cells in lectin-stained sections of the mouse descending colon by using a confocal laser scanning microscope. In the lower part of the crypt, the Golgi apparatus formed a dome- or globe-like structure in the supranuclear region. The wall of the dome had some holes, one of which usually faced toward the nucleus and others toward the apical cytoplasm. Mucous granules seemed to be initially released into the interior of the dome and transported toward the apical cytoplasm through the holes. In the upper part of the crypt, on the other hand, the Golgi apparatus formed a cup- or funnel-like structure with a larger opening toward the cell apex and a smaller opening toward the nucleus. A large mass of mucous granules occupied the inside of the cup to the apical cytoplasm. It is thought that the accumulation of mucous granules enlarges holes at the ceiling of the dome to form a large opening, which makes the configuration of the Golgi apparatus cup-shaped.
利用共聚焦激光扫描显微镜，在凝集素染色的小鼠降结肠切片中的杯状细胞中研究了高尔基体的三维结构。在隐窝下部，高尔基体在核上区形成圆顶状或球状结构。圆顶壁上有一些孔，其中一个通常朝向细胞核，其他孔朝向顶端细胞质。黏液颗粒似乎最初释放到圆顶内部，并通过这些孔向顶端细胞质运输。另一方面，在隐窝上部，高尔基体形成杯状或漏斗状结构，开口较大的一端朝向细胞顶端，开口较小的一端朝向细胞核。大量黏液颗粒占据杯内部直至顶端细胞质。据认为，黏液颗粒的积累会扩大圆顶顶部的孔以形成一个大开口，这使得高尔基体的形态呈杯状。
