基于核磁共振技术对山梨螺旋菌VKM Ac-1993(T)细胞壁阴离子聚合物的鉴定

NMR-based identification of cell wall anionic polymers of Spirilliplanes yamanashiensis VKM Ac-1993(T).

作者信息

Shashkov A S, Streshinskaya G M, Evtushenko L I, Naumova I B

机构信息

N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 117334, Russia.

出版信息

Carbohydr Res. 2001 Nov 21;336(3):237-42. doi: 10.1016/s0008-6215(01)00224-5.

DOI:10.1016/s0008-6215(01)00224-5

PMID:11705473

Abstract

The cell wall of Spirilliplanes yamanashiensis VKM Ac-1993(T) contains four anionic polymers, viz., three teichoic acids and a sugar-1-phosphate polymer. The following are the structures of the teichoic acids: poly[-6-beta-D-glucopyranosyl-(1-->2)-glycerol phosphate] (PI), 1,3-poly(glycerol phosphate) bearing N-acetyl-alpha-D-glucosamine residues at O-2 (70%) (PII), and poly[-6-N-acetyl-alpha-D-glucosaminyl-(1-->2)-glycerol phosphate] (PIII). The repeating unit of the fourth polymer (PIV) has the structure of -6-alpha-D-GlcpNAc-(1-->6)-alpha-D-GlcpNAc-1-P- with a 3-O-methyl-alpha-D-mannopyranosyl residues at position 3 of some 6-phosphorylated N-acetylglucosamine residues (50%). Polymers PI, PIII and PIV have not hitherto been found in prokaryotic cell walls.

摘要

山梨螺旋菌VKM Ac-1993(T)的细胞壁含有四种阴离子聚合物，即三种磷壁酸和一种糖-1-磷酸聚合物。以下是磷壁酸的结构：聚[-6-β-D-吡喃葡萄糖基-(1→2)-甘油磷酸]（PI）、在O-2位带有N-乙酰-α-D-葡萄糖胺残基（70%）的1,3-聚（甘油磷酸）（PII）和聚[-6-N-乙酰-α-D-葡萄糖胺基-(1→2)-甘油磷酸]（PIII）。第四种聚合物（PIV）的重复单元结构为-6-α-D-GlcpNAc-(1→6)-α-D-GlcpNAc-1-P-，在一些6-磷酸化的N-乙酰葡萄糖胺残基（50%）的3位带有一个3-O-甲基-α-D-甘露吡喃糖基残基。聚合物PI、PIII和PIV迄今尚未在原核细胞壁中发现。