通过碱性水解和高效液相色谱法灵敏测定淡水中溶解的色氨酸

Sensitive determination of dissolved tryptophan in freshwater by alkaline hydrolysis and HPLC.

作者信息

Wu F, Tanoue E

机构信息

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Science, Guiyang, 550002, China.

出版信息

Anal Sci. 2001 Sep;17(9):1063-6. doi: 10.2116/analsci.17.1063.

DOI:10.2116/analsci.17.1063

PMID:11708060

Abstract

A sensitive method involving alkaline hydrolysis and HPLC analysis has been developed to detect nanomolar levels of tryptophan dissolved in freshwater. The procedure includes 1) alkaline hydrolysis with nitrogen atmosphere in Teflon-lined Pyrex tubes with 4.2 M sodium hydroxide at 110 degrees C for 16 h, in which ascorbic acid was used as an antioxidant; 2) a determination step, in which tryptophan was separated efficiently from other amino acids by HPLC and measured by fluorescence. The mean recovery for tryptophan standard was 91.4% with an RSD of 3.3%. Using this method, dissolved tryptophan concentrations ranged from 10.91 to 41.83 nM in water samples from Lake Biwa, Japan.

摘要

已开发出一种涉及碱性水解和高效液相色谱（HPLC）分析的灵敏方法，用于检测溶解在淡水中纳摩尔水平的色氨酸。该程序包括：1）在衬有特氟龙的派热克斯玻璃管中，于氮气氛围下，用4.2 M氢氧化钠在110℃进行16小时的碱性水解，其中使用抗坏血酸作为抗氧化剂；2）测定步骤，通过HPLC将色氨酸与其他氨基酸有效分离，并通过荧光进行测量。色氨酸标准品的平均回收率为91.4%，相对标准偏差为3.3%。使用该方法，日本琵琶湖水样中溶解色氨酸的浓度范围为10.91至41.83 nM。