Fujimori F, Gunji W, Kikuchi J, Mogi T, Ohashi Y, Makino T, Oyama A, Okuhara K, Uchida T, Murakami Y
Department of Biological Science & Technology, Faculty of Industrial Science & Technology, Science University of Tokyo, Japan.
Biochem Biophys Res Commun. 2001 Nov 23;289(1):181-90. doi: 10.1006/bbrc.2001.5925.
Previous studies have indicated that Ess1/Pin1, a gene in the parvulin family of peptidyl-prolyl isomerases (PPIases), plays an important role in regulating the G(2)/M transition of the cell cycle by binding cell-cycle-regulating proteins in eukaryotic cells. Although the ess1 gene has been considered to be essential in yeast, we have isolated viable ess1 deletion mutants and demonstrated, via analysis of yeast gene expression profiles using microarray techniques, a novel regulatory role for ESS1 in the G(1) phase. Although the overall expression profiles in the tested strains (C110-1, W303, S288c, and RAY-3AD) were similar, marked changes were detected for a number of genes involved in the molecular action of ESS1. Among these, the expression levels of a cyclophilin A gene, also a member of the PPIase family, increased in the ess1 null mutant derived from C110-1. Subsequent treatment with cyclosporin A significantly retarded growth, which suggests that ESS1 and cyclophilin A are functionally linked in yeast cells and play important roles at the G(1) phase of the cell cycle.
先前的研究表明，Ess1/Pin1是肽基脯氨酰异构酶（PPIases）的小脯氨酸异构酶家族中的一个基因，它通过与真核细胞中的细胞周期调节蛋白结合，在调节细胞周期的G(2)/M转换中发挥重要作用。尽管ess1基因在酵母中被认为是必需的，但我们已经分离出了存活的ess1缺失突变体，并通过使用微阵列技术分析酵母基因表达谱，证明了ESS1在G(1)期具有新的调节作用。尽管测试菌株（C110-1、W303、S288c和RAY-3AD）的总体表达谱相似，但检测到许多参与ESS1分子作用的基因有明显变化。其中，同样属于PPIase家族成员的亲环蛋白A基因在源自C110-1的ess1缺失突变体中的表达水平升高。随后用环孢素A处理显著延缓了生长，这表明ESS1和亲环蛋白A在酵母细胞中功能相关，并在细胞周期的G(1)期发挥重要作用。
