van Harmelen J, Williamson C, Kim B, Morris L, Carr J, Karim S S, McCutchan F
Department of Medical Virology, University of Cape Town, Observatory, Cape Town, South Africa, 7925.
AIDS Res Hum Retroviruses. 2001 Nov 1;17(16):1527-31. doi: 10.1089/08892220152644232.
Four full-length genome subtype C sequences from South Africa, three of which are being used for vaccine development, were characterized. Three isolates were obtained from recently infected individuals in KwaZulu/Natal: Du151, Du422, and Du179. A fourth isolate, CTSc2, was obtained from an individual residing in Cape Town. All four strains used the CCR5 coreceptor, although Du179 also used CXCR4. The four isolates clustered within subtype C, but the three Du isolates formed a subcluster with a bootstrap value of 100%, with CTSc2 outside the subcluster. None of the strains showed evidence of intersubtype recombination, as expected from the predominance of subtype C in South Africa. All 4 isolates had a 16-amino acid truncation on the 3' end of the Rev protein, identified in other subtype C isolates. Like many other subtype C strains, Du151, Du422, and Du179 had three NF-kappa B-binding sites in the LTR; however, CTSc2 had only two.
对来自南非的4条C亚型全长基因组序列进行了特征分析，其中3条正用于疫苗研发。3株分离株来自夸祖鲁/纳塔尔省近期感染的个体：Du151、Du422和Du179。第4株分离株CTSc2来自开普敦的一名个体。所有4株毒株均使用CCR5共受体，不过Du179也使用CXCR4。这4株分离株聚集在C亚型内，但3株Du分离株形成一个自展值为100%的亚群，CTSc2在该亚群之外。正如南非C亚型占主导地位所预期的那样，没有一株毒株显示出亚型间重组的迹象。所有4株分离株在Rev蛋白的3'端都有一个16个氨基酸的截短，这在其他C亚型分离株中也有发现。与许多其他C亚型毒株一样，Du151、Du422和Du179在长末端重复序列（LTR）中有3个NF-κB结合位点；然而，CTSc2只有2个。
