Sato C, Kreider J W, Banks J, Garlick S, Davidson E A
Cancer Res. 1975 Sep;35(9):2532-9.
B16 melanoma cells were treated in culture with 5-bromo-2-deoxyuridine. The cell-associated and released proteoglycans and sialoglycopeptides were compared to those of control cultures treated with thymidine. The 5-bromo-2-deoxyuridine-treated cultures showed a marked reduction in the proportion of cell-associated proteoglycans and sialoglycopeptides, an increase in the synthesis of hyaluronic acid, the absence of high-molecular-weight chondroitin sulfate, and the presence of increased amounts of heparan sulfate in the media. In addition, the 5-bromo-2-deoxyuridine-treated cells had a higher DNA content and were larger than controls.
用5-溴-2-脱氧尿苷在培养中处理B16黑色素瘤细胞。将细胞相关的和释放的蛋白聚糖及唾液酸糖肽与用胸苷处理的对照培养物进行比较。经5-溴-2-脱氧尿苷处理的培养物显示细胞相关蛋白聚糖和唾液酸糖肽的比例显著降低，透明质酸合成增加，不存在高分子量硫酸软骨素，且培养基中硫酸乙酰肝素含量增加。此外，经5-溴-2-脱氧尿苷处理的细胞DNA含量更高，且比对照细胞更大。
