通过放射免疫分析法特异性测定女性血浆中的睾酮：一种适用于常规临床实验室的快速可靠方法。

Specific determination of testosterone in female plasma by radioimmunoassay: a rapid and reliable procedure for the routine clinical laboratory.

作者信息

Joyce B G, Fahmy D, Hillier S G

出版信息

Clin Chim Acta. 1975 Jul 23;62(2):231-8. doi: 10.1016/0009-8981(75)90232-6.

Abstract

A rapid and reliable procedure for the specific determination of testosterone in female plasma is described. The assay entails a simple biphasic solvent partitioning of plasma extracts in order to remove cross-reacting steroids before quantitation of testosterone by radioimmunoassay using an antiserum raised against testosterone-11alpha-hemisuccinyl-bovine serum albumin. "Testosterone' values obtained in this way are in excellent agreeement with those obtained following thin-layer chromatographic purification of plasma extracts. However, the present procedure is easier, faster and cheaper to perform, and enables a single technician to assay 32 females plasma samples (in duplicate) in one working day.

摘要

本文描述了一种快速可靠的特异性测定女性血浆中睾酮的方法。该测定方法包括对血浆提取物进行简单的双相溶剂分配，以便在使用针对睾酮-11α-半琥珀酰-牛血清白蛋白产生的抗血清通过放射免疫测定法定量睾酮之前去除交叉反应性类固醇。以这种方式获得的“睾酮”值与血浆提取物经薄层色谱纯化后获得的值高度一致。然而，目前的方法操作起来更简便、更快且更便宜，并且一名技术人员在一个工作日内能够检测32份女性血浆样本（一式两份）。