Llewelyn D E, Hillier S G, Read G F
Steroids. 1977 Mar;29(3):417-26. doi: 10.1016/0039-128x(77)90010-1.
Multivariable calibration curves have been used to enable testosterone and 5alpha-dihydrotestosterone to be assayed directly in plasma extracts without further pre-purification of the sample. Two antisera were used, both with relatively high, but different affinities for the substances measured, and with relatively low affinity towards all other substances tested. The antisera were obtained from rabbits immunized against testosterone-3-BSA and 5alpha-dihydrotestosterone-3-BSA. The technique was of adequate precision, accuracy and specificity. The last was examined by comparison of values obtained by the present method and those obtained following pre-purification by thin layer chromatography.
多变量校准曲线已被用于直接测定血浆提取物中的睾酮和5α-二氢睾酮，而无需对样品进行进一步的预纯化。使用了两种抗血清，它们对所测物质的亲和力相对较高但不同，而对所有其他测试物质的亲和力相对较低。这些抗血清是从用睾酮-3-牛血清白蛋白和5α-二氢睾酮-3-牛血清白蛋白免疫的兔子中获得的。该技术具有足够的精密度、准确度和特异性。通过比较本方法获得的值与薄层色谱预纯化后获得的值来检验特异性。
