de Oliveira P T, Jaeger M M, Miyagi S P, Jaeger R G
Department of Oral Pathology, School of Dentistry, University of São Paulo, SP, Brazil.
Virchows Arch. 2001 Oct;439(4):571-8. doi: 10.1007/s004280000380.
We have already demonstrated that a reconstituted basement membrane (Matrigel) is a key modulator of morphogenetic changes and cytodifferentiation of pleomorphic adenoma cells in culture. Myoepithelioma is considered to be a neoplasm closely related to pleomorphic adenoma and should experience similar induction processes. Thus, the aim of this study was to investigate whether Matrigel would influence myoepithelioma cells. We used a cell line derived from a human salivary gland plasmacytoid myoepithelioma (M1 cells) grown in a three-dimensional preparation of Matrigel. Phenotype differences were assessed using conventional light microscopy technique (haematoxylin and eosin) and phase and differential interference contrast (Nomarski). Immunofluorescence was carried out to detect smooth-muscle actin, laminin and type-IV collagen. M1 cells exhibited all proteins studied, showing a myoepithelial differentiation. M1 cells grown inside Matrigel presented morphological changes and changes in smooth-muscle actin status. By growing M1 cells inside Matrigel, it was possible to reproduce the tumour architecture with no duct-like structures. Based on our findings, we suggest that myoepithelioma would be derived from a cell with a commitment to myoepithelial differentiation. We also suggest that the mechanical properties of the matrix environment will likely regulate smooth-muscle actin expression in myoepithelioma.
我们已经证明，重组基底膜（基质胶）是培养中多形性腺瘤细胞形态发生变化和细胞分化的关键调节因子。肌上皮瘤被认为是一种与多形性腺瘤密切相关的肿瘤，应该经历类似的诱导过程。因此，本研究的目的是调查基质胶是否会影响肌上皮瘤细胞。我们使用了一种源自人涎腺浆细胞样肌上皮瘤的细胞系（M1细胞），在基质胶的三维制剂中培养。使用传统光学显微镜技术（苏木精和伊红）以及相差和微分干涉对比（诺马斯基）评估表型差异。进行免疫荧光检测平滑肌肌动蛋白、层粘连蛋白和IV型胶原。M1细胞呈现出所有研究的蛋白质，显示出肌上皮分化。在基质胶内生长的M1细胞呈现出形态变化和平滑肌肌动蛋白状态的变化。通过在基质胶内培养M1细胞，可以重现没有导管样结构的肿瘤结构。基于我们的发现，我们认为肌上皮瘤可能源自具有肌上皮分化倾向的细胞。我们还认为，基质环境的力学性质可能会调节肌上皮瘤中平滑肌肌动蛋白的表达。
