Li S, Fang W, Zhong H
Department of Pathology, Beijing Medical University, Beijing 100083.
Zhonghua Yi Xue Za Zhi. 1999 Sep;79(9):708-10.
To investigate the correlation of a newly-defined tumor metastasis suppressor gene KAI1/CD82 and metastatic potential of human cancer cell lines.
Expressions of KAI1/CD82 in eight human cancer cell lines of prostate, lung and melanoma with different metastasis potential were studied by Northern blot hybridization and the mutation were detected by PCR-SSCP.
Expressions of KAI1 mRNA decreased in human prostate cancer cell lines PC3 and PC3M, and lung cancer cell line PG which were metastatic cancer cell lines (integral light density values: 0.0319, 0.0266 and 0.0549, respectively). Expression of KAI1 mRNA showed high level in PAa, a human lung cancer cell line with no metastasis (integral light density value: 0.7313). In human melanoma cell lines with different metastasis potential (WM35, WM1341b, WM983a and WM451), expression of KAI1 showed moderate expression (integral light density values are 0.1798, 0.1582, 0.1501, 0.1800 respectively). PCR-SSCP analysis indicated the existence of band shift in exon 7 of KAI1 gene in PC3, PG and PC3M.
Decreased KAI1/CD82 expression may be associated with enhanced metastatic potential in prostate cancer cell lines and lung cancer cell line other than melanoma cell lines. The decreased expression may be partially nelaled with mutation of KAI1 gene.
研究新定义的肿瘤转移抑制基因KAI1/CD82与人类癌细胞系转移潜能的相关性。
采用Northern印迹杂交法研究KAI1/CD82在8种具有不同转移潜能的人前列腺癌、肺癌和黑色素瘤细胞系中的表达情况，并用PCR-SSCP法检测其突变情况。
KAI1 mRNA在转移性癌细胞系人前列腺癌细胞系PC3和PC3M以及肺癌细胞系PG中表达降低（积分光密度值分别为0.0319、0.0266和0.0549）。KAI1 mRNA在无转移的人肺癌细胞系PAa中表达水平较高（积分光密度值为0.7313）。在具有不同转移潜能的人黑色素瘤细胞系（WM35、WM1341b、WM983a和WM451）中，KAI1表达呈中等水平（积分光密度值分别为0.1798、0.1582、0.1501、0.1800）。PCR-SSCP分析表明，PC3、PG和PC3M中KAI1基因第7外显子存在条带迁移。
KAI1/CD82表达降低可能与前列腺癌细胞系和肺癌细胞系（而非黑色素瘤细胞系）转移潜能增强有关。其表达降低可能部分与KAI1基因突变有关。
