烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶组分p47（吞噬细胞氧化酶）和p40（吞噬细胞氧化酶）通过其PX结构域与埃兹蛋白结合。
The NADPH oxidase components p47(phox) and p40(phox) bind to moesin through their PX domain.
作者信息
Wientjes F B, Reeves E P, Soskic V, Furthmayr H, Segal A W
机构信息
Centre for Molecular Medicine, University College London, 5 University Street, London WC1E 6JJ, United Kingdom.
出版信息
Biochem Biophys Res Commun. 2001 Nov 30;289(2):382-8. doi: 10.1006/bbrc.2001.5982.
The NADPH oxidase of phagocytes is a membrane-bound heterodimeric flavocytochrome which catalyses the transfer of electrons from NADPH in the cytoplasm to oxygen in the phagosome. A number of cytosolic proteins are involved in its activation/deactivation: p47phox, p67phox, p40phox and the small GTP-binding protein, rac. The cytosolic phox proteins interact with the cytoskeleton in human neutrophils and, in particular, an interaction with coronin has been reported (Grogan A., Reeves, E., Keep, N. H., Wientjes, F., Totty, N., Burlingame, N. L., Hsuan, J., and Segal, A. W. (1997) J. Cell Sci. 110, 3071-3081). Here, we report on the interaction of another cytoskeletal protein, moesin, with the phox proteins. Moesin belongs to the ezrin-radixin-moesin family of F-actin-binding proteins and we show that it binds to p47phox and p40phox in a phosphoinositide-dependent manner. Furthermore, we show that its N-terminal part binds to the PX domain of p47phox and p40phox.
吞噬细胞的NADPH氧化酶是一种膜结合的异二聚体黄素细胞色素，它催化电子从细胞质中的NADPH转移至吞噬小体中的氧气。一些胞质蛋白参与其激活/失活过程：p47phox、p67phox、p40phox以及小GTP结合蛋白rac。胞质phox蛋白在人类中性粒细胞中与细胞骨架相互作用，尤其是据报道它与冠蛋白存在相互作用（Grogan A.、Reeves E.、Keep N. H.、Wientjes F.、Totty N.、Burlingame N. L.、Hsuan J.和Segal A. W.（1997年）《细胞科学杂志》110卷，3071 - 3081页）。在此，我们报道另一种细胞骨架蛋白埃兹蛋白与phox蛋白的相互作用。埃兹蛋白属于F - 肌动蛋白结合蛋白的埃兹蛋白 - 根蛋白 - 埃兹蛋白家族，并且我们表明它以磷酸肌醇依赖性方式与p47phox和p40phox结合。此外，我们表明其N端部分与p47phox和p40phox的PX结构域结合。
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