Sun J, Liu C
PLA Navy General Hospital, Beijing 100037.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1998 Aug;33(4):196-8.
To create a cytological model of spiral ganglion neurons in postnatal mammals in vitro.
The primary culture of spiral ganglion neurons (SGN) was carried out with seven-day postnatal Wistar rats. The process of cellular growth and differentiation of SGN were observed by fluorescent microscope and inverted/phase contrast microscopy. Immunocytochemical identification was performed on the cultured SGN of Wistar rats by methods of S-P and the monoclonal antibody of neurofilament protein (NFP-McAb).
The present work showed that the dissociated SGN of Wistar rat could survive well and had a normal phenotypic differentiation in vitro. The cell amount and surviving period of SGN were able to meet the requirements of cytological experiments. The stable neuronal plasticity of SGN existed in the postnatal mammal under the present experimental conditions.
This method extends the material sources of inner ear tissue culture and provides a useful approach to the neurobiologic studies on the peripheral auditory system in vitro.
在体外建立出生后哺乳动物螺旋神经节神经元的细胞学模型。
采用出生7天的Wistar大鼠进行螺旋神经节神经元（SGN）的原代培养。通过荧光显微镜和倒置/相差显微镜观察SGN的细胞生长和分化过程。采用S-P法和神经丝蛋白单克隆抗体（NFP-McAb）对Wistar大鼠培养的SGN进行免疫细胞化学鉴定。
本研究表明，Wistar大鼠解离的SGN在体外能良好存活并具有正常的表型分化。SGN的细胞数量和存活时间能够满足细胞学实验的要求。在本实验条件下，出生后哺乳动物的SGN存在稳定的神经元可塑性。
该方法拓展了内耳组织培养的材料来源，为体外周围听觉系统的神经生物学研究提供了一种有用的方法。
