微波热疗对慢性肢体淋巴水肿患者全身及局部浸润淋巴细胞的影响。

Effects of microwave heating on systemic and local infiltrating lymphocytes in patients with chronic limb lymphedema.

作者信息

Cao W, Chang T, Gan J

机构信息

Department of Plastic & Reconstructive Surgery, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.

出版信息

Chin Med J (Engl). 1999 Sep;112(9):822-7.

PMID:11717954

Abstract

OBJECTIVES

To observe the characteristics of lymphocyte phenotypes in systemic and local skin and to evaluate the effects of microwave heating and bandaging treatment on chronic limb lymphedema.

METHODS

Totally 27 patients with lymphedema and 10 normal subjects were examined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) and avidin-biotin-peroxidase (ABC) immunohistochemistry for the observation of systemic lymphocyte phenotypes and inflammatory cell infiltration of skin tissues.

RESULTS

In the peripheral blood of patients with chronic limb lymphedema, the number of CD4 T lymphocytes and the ratio of CD4/CD8 decreased, while the number of CD8 T lymphocytes increased. Obvious dermal perivascular infiltration of T lymphocytes was also observed. After two courses of microwave heating and bandaging treatment, the number of CD4 T lymphocytes augmented and the decreased CD4/CD8 ratio returned to normal, and the number of CD8 T lymphocytes reduced. The perivascular T lymphocyte infiltration in the dermis resolved and the number of macrophages elevated.

CONCLUSION

Microwave heating and bandaging treatment can regulate the imbalance of systemic and local immunity in patients with chronic lymphedema.

摘要

目的

观察全身及局部皮肤淋巴细胞表型特征，评估微波加热联合绷带治疗对慢性肢体淋巴水肿的影响。

方法

采用碱性磷酸酶抗碱性磷酸酶（APAAP）和抗生物素蛋白-生物素-过氧化物酶（ABC）免疫组化法，对27例淋巴水肿患者及10例正常对照者进行检测，观察全身淋巴细胞表型及皮肤组织炎性细胞浸润情况。

结果

慢性肢体淋巴水肿患者外周血中CD4⁺ T淋巴细胞数量减少，CD4⁺/CD8⁺比值降低，CD8⁺ T淋巴细胞数量增加，同时可见明显的真皮血管周围T淋巴细胞浸润。经过两个疗程的微波加热联合绷带治疗后，CD4⁺ T淋巴细胞数量增加，降低的CD4⁺/CD8⁺比值恢复正常，CD8⁺ T淋巴细胞数量减少，真皮血管周围T淋巴细胞浸润消退，巨噬细胞数量增加。