Wang Y, Wang J, Gao Y
Department of Operative Dentistry and Endodontics, Peking University School of Stomatology, Beijing 100081, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2001 Jul;36(4):259-61.
To observe and compare the expression pattern of Msx-1, Msx-2 mRNA during the different stages of hard tissue formation in the first mandibular molar of mouse and investigate the relationship between the two genes.
First mandibular molar germs from 1, 3, 7 and 14-days old mouse were separated and reverse transcription-polymerase chain reaction was performed on the total RNA of them using Msx-1, Msx-2 specific primers separately.
Expression of both genes were detected during the different stages of hard tissue formation in the mouse first mandibular molars, but there was some interesting differences in the quantitiy between the two genes. Msx-1 transcripts appeared at the 1 day postnatally, and increase through 3 day, 7 day, then maximally expressed at 14 days postnatally; while Msx-2 mRNA was seen and expressed maximally at the 3 days postnatally, then there was a gradual reduction at 7 days, and 14 days postnatally.
The homeobox gene Msx-1, Msx-2 may play a role in the events of the hard tissue formation. The complementary expression pattern of them during the specific stage of hard tissue formation indicates that there may be some functional redundancy between them during the biomineralization.
观察并比较Msx-1、Msx-2 mRNA在小鼠下颌第一磨牙硬组织形成不同阶段的表达模式，探讨这两个基因之间的关系。
分离1、3、7和14日龄小鼠的下颌第一磨牙牙胚，分别用Msx-1、Msx-2特异性引物对其总RNA进行逆转录聚合酶链反应。
在小鼠下颌第一磨牙硬组织形成的不同阶段均检测到这两个基因的表达，但二者在表达量上存在一些有趣的差异。Msx-1转录本在出生后1天出现，在3天、7天增加，然后在出生后14天达到最大表达；而Msx-2 mRNA在出生后3天出现并达到最大表达，然后在出生后7天和14天逐渐减少。
同源框基因Msx-1、Msx-2可能在硬组织形成过程中发挥作用。它们在硬组织形成特定阶段的互补表达模式表明，在生物矿化过程中它们之间可能存在一些功能冗余。
