Peri S, Pandey A
Protein Interaction Laboratory, Center for Experimental Bioinformatics, University of Southern Denmark, Odense M, Denmark.
Trends Genet. 2001 Dec;17(12):685-7. doi: 10.1016/s0168-9525(01)02493-3.
More than two decades ago Marilyn Kozak proposed the scanning model of translation initiation, whereby translation is initiated at the first AUG codon that is in a particular context. In this article, we re-examine the context of initiator codons using a large dataset of curated human transcripts. We find that more than 40% of transcripts contain AUG codons upstream of the actual start codon and that most authentic AUGs contain three or more mismatches from the consensus sequence, CCACCaugG. Also, in a large fraction of transcripts, the sequences surrounding the initiator codon deviate more from the consensus than those surrounding upstream AUGs, indicating that translation initiation from downstream AUGs is more common than generally believed.
二十多年前，玛丽莲·科扎克提出了翻译起始的扫描模型，即翻译在特定语境中的第一个AUG密码子处起始。在本文中，我们使用一个经过整理的人类转录本大型数据集重新审视起始密码子的语境。我们发现，超过40%的转录本在实际起始密码子上游含有AUG密码子，并且大多数真实的AUG与共有序列CCACCaugG有三个或更多错配。此外，在很大一部分转录本中，起始密码子周围的序列比上游AUG周围的序列更偏离共有序列，这表明从下游AUG起始翻译比普遍认为的更为常见。
