Kong J R, Kim S K, Moon B J, Kim S J, Kim B H
Department of Chemistry, Center for Integrated Molecular Systems, Pohang University of Science and Technology, Pohang, Korea.
Nucleosides Nucleotides Nucleic Acids. 2001 Oct-Nov;20(10-11):1751-60. doi: 10.1081/NCN-100107187.
We have prepared four diastereomers of TT dinucleotides containing an isoxazoline moiety and four dodecanucleotides incorporating these TT dinucleotides in the middle of the DNA sequence. We also have determined the melting temperatures of these modified oligonucleotides (Tm values) by measuring change in UV absorbance.
我们制备了四种含有异恶唑啉部分的TT二核苷酸非对映异构体，以及四种在DNA序列中间掺入这些TT二核苷酸的十二核苷酸。我们还通过测量紫外吸光度的变化测定了这些修饰寡核苷酸的解链温度（Tm值）。
