单核细胞增生李斯特菌噬菌体A118新型DNA:m4C甲基转移酶M.LmoA118I的克隆与特性分析，它是M.NgoMXV的紧密同源物
Cloning and characterization of M.LmoA118I, a novel DNA:m4C methyltransferase from the Listeria monocytogenes phage A118, a close homolog of M.NgoMXV.
作者信息
Bujnicki J M, Radlinska M
机构信息
Bioinformatics Laboratory, International Institute of Molecular and Cell Biology, Warsaw, Poland.
出版信息
Acta Microbiol Pol. 2001;50(2):155-60.
A homolog of M.NgoMXV DNA:m4C methyltransferase has been identified among the open reading frames deduced from the genomic sequence of Listeria monocytogenes phage A118 [Loessner et al., 2000]. The gene coding for this putative protein has been cloned in Escherichia coli and its enzymatic activity in vivo in this host have been analyzed. Remarkably, despite M.NgoMXV and M.LmoA118I exhibit high sequence similarity (58% identical and 19% conservatively substituted residues), their target preferences differ: both proteins exhibit "relaxed" sequence specificity, but while M.LmoA118I more efficiently methylates GGCC sites, it seems to target only a subset of CCWGG sites methylated by M.NgoMXV.
在从单核细胞增生李斯特菌噬菌体A118的基因组序列推导的开放阅读框中，已鉴定出一种与M.NgoMXV DNA:m4C甲基转移酶同源的蛋白[洛斯纳等人，2000年]。编码这种假定蛋白的基因已在大肠杆菌中克隆，并分析了其在该宿主中的体内酶活性。值得注意的是，尽管M.NgoMXV和M.LmoA118I表现出高度的序列相似性（58%相同且19%保守取代残基），但它们的靶标偏好不同：这两种蛋白都表现出“宽松”的序列特异性，但M.LmoA118I更有效地甲基化GGCC位点，它似乎只靶向M.NgoMXV甲基化的CCWGG位点的一个子集。
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