Hataya T, Arimoto R, Suda N, Uyeda I
Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Arch Virol. 2001 Oct;146(10):1935-48. doi: 10.1007/s007050170043.
The 3'-terminal sequence of hop mosaic virus (HpMV) genomic RNA was determined. A cDNA of approximately 1.8 kbp was amplified from the HpMV genome by 3' RACE using a degenerate primer, which was designed to anneal to the overlapping region of open reading frames (ORFs) 2 and 3 of eight carlavirus genomes. The sequence contained three ORFs, encoding proteins of 7-, 34-, and 11-kDa, which corresponded to ORFs 4, 5, and 6 of the carlavirus genome, respectively. The amino acid sequence of ORF 5, encoding the coat protein (CP) of HpMV, shows the highest identity (67%) to that of Hop latent virus (HpLV). The HpMV CP N-terminal sequence differs from that of HpLV, but the central and C-terminal sequences of the CP of both viruses are similar. The sequence similarity possibly causes the cross-reaction of heterologous antibodies of HpMV and HpLV. Phylogenetic analyses based on the CP amino acid and 3' non-coding region sequences indicate close relationships among HpMV, HpLV, and Potato virus M. We report here the first molecular characterization of HpMV genomic RNA.
测定了啤酒花花叶病毒（HpMV）基因组RNA的3'末端序列。使用简并引物通过3'RACE从HpMV基因组中扩增出一个约1.8kbp的cDNA，该引物设计用于与8种香石竹潜隐病毒基因组的开放阅读框（ORF）2和3的重叠区域退火。该序列包含三个ORF，分别编码7kDa、34kDa和11kDa的蛋白质，它们分别对应于香石竹潜隐病毒基因组的ORF4、5和6。编码HpMV外壳蛋白（CP）的ORF5的氨基酸序列与啤酒花潜隐病毒（HpLV）的氨基酸序列具有最高的同一性（67%）。HpMV CP的N端序列与HpLV不同，但两种病毒CP的中央和C端序列相似。这种序列相似性可能导致HpMV和HpLV的异源抗体发生交叉反应。基于CP氨基酸和3'非编码区序列的系统发育分析表明，HpMV、HpLV和马铃薯病毒M之间关系密切。我们在此报告HpMV基因组RNA的首次分子特征。
