Zheng H-Y, Chen J, Adams M J, Chen J-P
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, People's Republic of China.
Arch Virol. 2006 Aug;151(8):1667-72. doi: 10.1007/s00705-006-0727-x. Epub 2006 Feb 20.
The complete sequence of an isolate of Narcissus common latent virus (NCLV) from Zhangzhou city, Fujian, China was determined from amplified fragments of purified viral RNA. Excluding the poly(A) tail, the genomic RNA of NCLV was 8539 nucleotides (nt) long and had the typical organization for a member of the genus Carlavirus. The most closely related species were Potato virus M, Hop latent virus and Aconitum latent virus, which had 58-59% nt identity to NCLV in their entire genomes. These relationships were confirmed by a phylogenetic analysis using a composite nucleotide alignment of all the open reading frames.
通过对纯化病毒RNA的扩增片段进行测定，确定了来自中国福建省漳州市的水仙普通潜伏病毒（NCLV）分离株的完整序列。去除多聚（A）尾后，NCLV的基因组RNA长度为8539个核苷酸（nt），具有香石竹潜病毒属成员的典型结构。与之亲缘关系最近的物种是马铃薯M病毒、啤酒花潜伏病毒和乌头潜伏病毒，它们在全基因组上与NCLV的核苷酸同一性为58 - 59%。通过对所有开放阅读框的复合核苷酸比对进行系统发育分析，证实了这些关系。
