Becker K, Keller B, von Eiff C, Brück M, Lubritz G, Etienne J, Peters G
Institute of Medical Microbiology, University of Münster, 48149 Münster, Germany.
Appl Environ Microbiol. 2001 Dec;67(12):5551-7. doi: 10.1128/AEM.67.12.5551-5557.2001.
Staphylococcal food poisoning (SFP) caused by enterotoxigenic staphylococci is one of the main food-borne diseases. In contrast to Staphylococcus aureus, a systematic screening for the enterotoxins has not yet been performed on the genomic level for the coagulase-positive species S. intermedius. Therefore, the enterotoxigenic potential of 281 different veterinary (canine, n = 247; equine, n = 23; feline, n = 9; other, n = 2) and 11 human isolates of S. intermedius was tested by using a multiplex PCR DNA-enzyme immunoassay system targeting the staphylococcal enterotoxin genes sea, seb, sec, sed, and see. Molecular results were compared by in vitro testing of enterotoxin production by two immunoassays. A total of 33 (11.3%) S. intermedius isolates, including 31 (12.6%) canine isolates, 1 equine isolate, and 1 human isolate, tested positive for the sec gene. In vitro production of the respective enterotoxins was detected in 30 (90.9%) of these isolates by using immunological tests. In contrast, none of 65 veterinary specimen-derived isolates additionally tested and comprising 13 (sub)species of coagulase-negative staphylococci were found to be enterotoxigenic. This study shows on both molecular and immunological levels that a substantial number of S. intermedius isolates harbor the potential for enterotoxin production. Since evidence for noninvasive zoonotic transmission of S. intermedius from animal hosts to humans has been documented, an enterotoxigenic role of this microorganism in SFP via contamination of food products may be assumed.
由产肠毒素葡萄球菌引起的葡萄球菌食物中毒（SFP）是主要的食源性疾病之一。与金黄色葡萄球菌不同，尚未在基因组水平上对中间葡萄球菌这种凝固酶阳性菌进行肠毒素的系统筛查。因此，使用针对葡萄球菌肠毒素基因sea、seb、sec、sed和see的多重PCR DNA酶免疫分析系统，对281株不同的中间葡萄球菌兽医分离株（犬源，n = 247；马源，n = 23；猫源，n = 9；其他，n = 2）和11株人源分离株的产肠毒素潜力进行了检测。通过两种免疫分析对肠毒素产生进行体外检测，比较分子检测结果。共有33株（11.3%）中间葡萄球菌分离株sec基因检测呈阳性，包括31株（12.6%）犬源分离株、1株马源分离株和1株人源分离株。通过免疫检测在这些分离株中的30株（90.9%）中检测到了相应肠毒素的体外产生。相比之下，另外检测的65株兽医标本来源的分离株（包括13种凝固酶阴性葡萄球菌（亚）种）均未发现具有产肠毒素能力。这项研究在分子和免疫水平上均表明，大量中间葡萄球菌分离株具有产生肠毒素的潜力。由于已有文献记载中间葡萄球菌从动物宿主向人类的非侵入性人畜共患病传播证据，因此可以推测这种微生物通过污染食品在SFP中具有产肠毒素作用。
