Hritcu Ozana M, Schmidt Vanessa M, Salem Shebl E, Maciuca Iuliana E, Moraru Ramona F, Lipovan Irina, Mareş Mihai, Solcan Gheorghe, Timofte Dorina
Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Iaşi, Romania.
Department of Small Animal Clinical Science, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Wirral, United Kingdom.
Front Vet Sci. 2020 Jul 21;7:414. doi: 10.3389/fvets.2020.00414. eCollection 2020.
The objective of this study was to compare virulence and resistance factors of mucosal and cutaneous staphylococci from dogs with pyoderma in the UK and Romania, two countries with different approaches to antimicrobial use in companion animals. Staphylococcal isolates ( = 166) identified to the species level as being or coagulase negative (CoNS) were analyzed for their antimicrobial resistance (AMR) profile and presence of resistance and virulence genes. Of the investigated isolates, 26 were methicillin-resistant (MRSP), 89 were methicillin-susceptible (MSSP) and 51 were coagulase negative staphylococci (CoNS). A significantly larger number of isolates originating from Romania were resistant to clindamycin, tetracycline, and chloramphenicol compared to the UK isolates ( < 0.05). Resistance to amoxicillin-clavulanic acid, gentamicin, and trimethoprim-sulphamethoxazole was more evident in UK isolates. Fusidic acid resistance was common in spp. isolates from both countries. Most isolates carried virulence factors associated with (exfoliative toxin) and (leucocidin) genes. All MRSP UK isolates exhibited fusidic acid resistance genes whilst this was very rare in the MRSP isolates from Romania. The chlorhexidine resistance gene was frequently identified in CoNS isolates from the UK ( < 0.001). The current study documented differences in antimicrobial resistance profiles of spp. isolates from dogs in two geographical locations in Europe, which could reflect differences in antimicrobial prescribing patterns. The study also highlights the need for further studies and interventions on antimicrobial use, prescribing patterns and AMR surveillance in companion animals in Romania.
本研究的目的是比较英国和罗马尼亚患有脓皮病犬的黏膜和皮肤葡萄球菌的毒力和耐药因子，这两个国家在伴侣动物抗菌药物使用方面采取了不同的方法。对鉴定到种水平为金黄色葡萄球菌或凝固酶阴性葡萄球菌（CoNS）的葡萄球菌分离株（n = 166）进行了抗菌药物耐药性（AMR）谱分析以及耐药基因和毒力基因检测。在所研究的分离株中，26株为耐甲氧西林金黄色葡萄球菌（MRSP），89株为甲氧西林敏感金黄色葡萄球菌（MSSP），51株为凝固酶阴性葡萄球菌（CoNS）。与英国分离株相比，源自罗马尼亚的分离株对克林霉素、四环素和氯霉素耐药的数量显著更多（P < 0.05）。英国分离株对阿莫西林 - 克拉维酸、庆大霉素和甲氧苄啶 - 磺胺甲恶唑的耐药性更明显。两国的金黄色葡萄球菌分离株中对夫西地酸耐药都很常见。大多数分离株携带与金黄色葡萄球菌（剥脱毒素）和杀白细胞素相关的毒力因子基因。所有英国的MRSP分离株均表现出夫西地酸耐药基因，而在罗马尼亚的MRSP分离株中这种情况非常罕见。在英国的CoNS分离株中经常鉴定出洗必泰耐药基因（P < 0.001）。本研究记录了欧洲两个地理位置犬的金黄色葡萄球菌分离株抗菌药物耐药谱的差异，这可能反映了抗菌药物处方模式的差异。该研究还强调了对罗马尼亚伴侣动物抗菌药物使用、处方模式和AMR监测进行进一步研究和干预的必要性。
