Sloma A, Smith I
Mol Gen Genet. 1979 Sep;175(2):113-20. doi: 10.1007/BF00425526.
We have analyzed the RNA synthesized during spore germination in Bacillus subtilis. Early in germination there is little incorporation of [3H]uridine into RNA. A large increase in incorporation into RNA was found at 45--60 min into germination which was in part due to increases in the specific activity of the UTP pool. When corrected for specific activity changes, the instantaneous rate of RNA synthesis showed a seven to tenfold increase between 30 and 45 min of germination. Polyacrylamide gel electrophoresis studies showed that the RNA synthesized during germination appeared very similar to the RNA made during vegetative growth. DNA-RNA hybridization studies indicated that mRNA and rRNA were synthesized throughout germination. Their relative proportions remained constant and were very similar to the composition of RNA synthesized during vegetative growth.
我们分析了枯草芽孢杆菌孢子萌发过程中合成的RNA。在萌发早期，[3H]尿苷掺入RNA的量很少。在萌发45 - 60分钟时，发现掺入RNA的量大幅增加，这部分是由于UTP池比活性的增加。校正比活性变化后，RNA合成的瞬时速率在萌发30至45分钟之间增加了7至10倍。聚丙烯酰胺凝胶电泳研究表明，萌发过程中合成的RNA与营养生长过程中合成的RNA非常相似。DNA - RNA杂交研究表明，在整个萌发过程中都合成了mRNA和rRNA。它们的相对比例保持恒定，并且与营养生长过程中合成的RNA组成非常相似。
