Azegami M, Ochi N, Yanagihara S, Ishii S
Microbios. 1978;23(92):73-81.
Mitomycin C induced a pyocinogenic Pseudomonas aeruginosa P15 to produce a bacteriolytic enzyme, PR1-lysozyme, together with pyocin R1. No significant accumulation of the enzyme was observed inside the induced cells. The enzyme was partially purified by acrinol treatment and Amberlie CG-50 column chromatography. The mode of action of the enzyme on the host bacterial cells as well as on Micrococcus lysodeikticus cells or peptidoglycan isolated from Salmonella typhimurium, was compared with that of hen egg-white lysozyme or phage lambda-lysozyme. It is suggested that PR1-lysozyme should be classified as a glycosidase, rather than an amidase or an endopeptidase.
丝裂霉素C诱导产绿脓菌素的铜绿假单胞菌P15产生一种溶菌酶PR1-溶菌酶，同时产生绿脓菌素R1。在诱导细胞内未观察到该酶的显著积累。通过吖啶醇处理和Amberlie CG-50柱色谱法对该酶进行了部分纯化。将该酶对宿主细菌细胞以及对溶壁微球菌细胞或从鼠伤寒沙门氏菌分离的肽聚糖的作用方式，与鸡蛋清溶菌酶或噬菌体λ-溶菌酶的作用方式进行了比较。结果表明，PR1-溶菌酶应归类为糖苷酶，而不是酰胺酶或内肽酶。
