基于高速光纤的偏振敏感光学相干断层扫描技术在体内烧伤深度测定中的应用

In vivo burn depth determination by high-speed fiber-based polarization sensitive optical coherence tomography.

作者信息

Park B H, Saxer C, Srinivas S M, Nelson J S, de Boer J F

机构信息

Beckman Laser Institute and Medical Clinic, University of California, 1002 Health Sciences Road East, Irvine, CA 92612, USA.

出版信息

J Biomed Opt. 2001 Oct;6(4):474-9. doi: 10.1117/1.1413208.

DOI:10.1117/1.1413208

PMID:11728208

Abstract

We report the first application of high-speed fiber-based polarization sensitive optical coherence tomography (PS-OCT) to image burned tissue in vivo. Thermal injury denatures collagen in skin and PS-OCT can measure the reduction in collagen birefringence using depth resolved changes in the polarization state of light propagated in, and reflected from, the tissue. Stokes vectors were calculated for each point in a scan and birefringence relative to incident polarization determined using four incident polarization states. Using a high-speed fiber-based PS-OCT system on rat skin burned for varying periods of time, a correlation between birefringence and actual burn depth determined by histological analysis was established. In conclusion, PS-OCT has potential use for noninvasive assessment of burn depth.

摘要

我们报告了首次将基于光纤的高速偏振敏感光学相干断层扫描（PS-OCT）应用于体内烧伤组织成像。热损伤使皮肤中的胶原蛋白变性，而PS-OCT可以利用在组织中传播并从组织反射的光的偏振态的深度分辨变化来测量胶原蛋白双折射的降低。为扫描中的每个点计算斯托克斯矢量，并使用四种入射偏振态确定相对于入射偏振的双折射。使用基于光纤的高速PS-OCT系统对不同烧伤时间的大鼠皮肤进行检测，建立了双折射与通过组织学分析确定的实际烧伤深度之间的相关性。总之，PS-OCT在烧伤深度的无创评估方面具有潜在应用价值。