Conformational and functional significance of residue proline 17 in chicken muscle adenylate kinase. - Suppr | 超能文献

Conformational and functional significance of residue proline 17 in chicken muscle adenylate kinase.

作者信息

Sheng X, Pan X, Wang C, Zhang Y, Jing G

机构信息

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, PR China.

出版信息

FEBS Lett. 2001 Nov 23;508(3):318-22. doi: 10.1016/s0014-5793(01)03092-7.

DOI:10.1016/s0014-5793(01)03092-7

Abstract

The effect of mutation proline 17 on the multiple conformations and catalytic function in chicken muscle adenylate kinase (AK) has been studied. The substitution of proline 17 with glycine or valine altered the distribution of multiple conformations. Compared with the wild-type enzyme, the P17G and P17V mutants contained decreased fraction of minor conformer from 18% to 9% and 11%, respectively. Due to the mutation, the enzyme showed lower secondary structural content, poorer affinity to substrates or substrate analogues, and reduced catalytic efficiency. The results revealed the significance of proline 17 in the conformation and function of AK.

摘要

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