与椰子和油棕枯萎病相关的韧皮部受限植鞭毛虫的动质体DNA小环具有双结构域结构。

Kinetoplast DNA minicircles of phloem-restricted Phytomonas associated with wilt diseases of coconut and oil palms have a two-domain structure.

作者信息

Dollet M, Sturm N R, Ahomadegbe J C, Campbell D A

机构信息

CIRAD, TA 30/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France.

出版信息

FEMS Microbiol Lett. 2001 Nov 27;205(1):65-9. doi: 10.1111/j.1574-6968.2001.tb10926.x.

DOI:10.1111/j.1574-6968.2001.tb10926.x

PMID:11728717

Abstract

We report the cloning and sequencing of the first minicircle from a phloem-restricted, pathogenic Phytomonas sp. (Hart 1) isolated from a coconut palm with hartrot disease. The minicircle possessed a two-domain structure of two conserved regions, each containing three conserved sequence blocks (CSB). Based on the sequence around CSB 3 from Hart 1, PCR primers were designed to allow specific amplification of Phytomonas minicircles. This primer pair demonstrated specificity for at least six groups of plant trypanosomatids and did not amplify from insect trypanosomatids. The PCR results were consistent with a two-domain structure for other plant trypanosomatids.

摘要

我们报告了从患有哈特罗病的椰子棕榈中分离出的韧皮部限制性致病植生滴虫（Hart 1）的首个微小环的克隆和测序。该微小环具有两个保守区域的双结构域结构，每个区域包含三个保守序列块（CSB）。基于Hart 1的CSB 3周围序列，设计了PCR引物以特异性扩增植生滴虫微小环。该引物对至少对六组植物锥虫显示出特异性，并且不会从昆虫锥虫中扩增。PCR结果与其他植物锥虫的双结构域结构一致。