Marché S, Roth C, Philippe H, Dollet M, Baltz T
Laboratoire de Biologie Moléculaire et d'Immunologie de Protozoaires Parasites, Université de Bordeaux II, URA 1637 CNRS, France.
Mol Biochem Parasitol. 1995 Apr;71(1):15-26. doi: 10.1016/0166-6851(95)00029-z.
The complete sequences of the genomic small subunit ribosomal RNA gene from two Phytomonas isolates: one associated with palm pathologies (P. cocos FGuiana) and one found in lactiferous plants with no apparent pathology (P. Euphorbe Senegal), were analyzed. Partial sequences from a number of other Phytomonas isolates were also determined. The sequences obtained were used to determine the phylogenetic relationships between Phytomonas and other trypanosomatids as well as within the genus Phytomonas. The analysis showed that the intraphloemic isolates associated with pathologies in palm trees formed a homogeneous group that diverged from the more heterogeneous group of non-pathogenic isolates found in latex plant. Sequence comparisons of the full and partial SSU rRNA gene, identified sequences which are specific to the genus Phytomonas and an EcoRI restriction nuclease site which specifically identifies the Phytomonas isolates associated with diseases in palm trees.
对来自两种植生滴虫分离株的基因组小亚基核糖体RNA基因的完整序列进行了分析：一种与棕榈树病变有关（圭亚那椰植生滴虫），另一种存在于无明显病变的乳汁植物中（塞内加尔大戟植生滴虫）。还测定了许多其他植生滴虫分离株的部分序列。所获得的序列用于确定植生滴虫与其他锥虫之间以及植生滴虫属内的系统发育关系。分析表明，与棕榈树病变相关的韧皮部内分离株形成了一个同质群体，该群体与在乳胶植物中发现的异质性更强的非致病分离株群体不同。对完整和部分小亚基核糖体RNA基因的序列比较，确定了植生滴虫属特有的序列以及一个EcoRI限制性核酸酶位点，该位点可特异性识别与棕榈树疾病相关的植生滴虫分离株。
