微小Tn5cyaA'的构建及其在鉴定支气管败血波氏杆菌中编码表面暴露蛋白和分泌蛋白的基因方面的应用。

Construction of mini-Tn5cyaA' and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica.

作者信息

Tu X, Nisan I, Miller J F, Hanski E, Rosenshine I

机构信息

Department of Molecular Genetics and Biotechnology, Faculty of Medicine, The Hebrew University, P.O. Box 12272, Jerusalem 91120, Israel.

出版信息

FEMS Microbiol Lett. 2001 Nov 27;205(1):119-23. doi: 10.1111/j.1574-6968.2001.tb10934.x.

DOI:10.1111/j.1574-6968.2001.tb10934.x

PMID:11728725

Abstract

A mini-Tn5 transposon derivative, mini-Tn5cyaA', has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA'). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA' translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.

摘要

构建了一种迷你Tn5转座子衍生物，即迷你Tn5cyaA'。它包含一个无启动子且核糖体结合位点缺陷的报告基因，该基因编码百日咳博德特氏菌腺苷酸环化酶毒素（CyaA'）的催化结构域。我们使用该系统对支气管败血博德特氏菌进行诱变，并开发了一种筛选方法来鉴定含有cyaA'翻译融合体的突变体。该系统用于鉴定编码表面暴露和分泌蛋白的支气管败血博德特氏菌基因。