Beall B
Centers for Disease Control and Prevention, Respiratory Diseases Laboratory Section, Atlanta, GA 30333, USA.
Res Microbiol. 1998 Mar;149(3):189-201. doi: 10.1016/s0923-2508(98)80079-x.
Two iron-regulated genes with deduced homology to TonB-dependent ferric siderophore receptors were cloned from Bordetella bronchiseptica by screening a library of TnphoA insertion mutants. bfrB and bfrC were iron-repressed in B. bronchiseptica by a Fur-dependent mechanism, and were expressed from promoters overlapped by potential Fur-binding sites. Both genes were highly conserved among Bordetella species and were also iron-regulated in Bordetella pertussis. bfrB and bfrC mutants of both species and a bfrB-bfrC double mutant of B. bronchiseptica had no discernible defects in utilization of known iron sources for Bordetella.
通过筛选TnphoA插入突变体文库，从支气管败血博德特氏菌中克隆出两个与TonB依赖性铁载体受体具有推导同源性的铁调节基因。bfrB和bfrC在支气管败血博德特氏菌中通过一种Fur依赖性机制被铁抑制，并从与潜在Fur结合位点重叠的启动子表达。这两个基因在博德特氏菌属中高度保守，在百日咳博德特氏菌中也受铁调节。两种菌的bfrB和bfrC突变体以及支气管败血博德特氏菌的bfrB - bfrC双突变体在利用博德特氏菌已知铁源方面没有明显缺陷。
