Luo K, Zhang L
Key Laboratory of Viral Hepatitis, Nanfang Hospital, Guangzhou 510515, China.
Chin Med J (Engl). 2001 Nov;114(11):1201-4.
To detect the virus in the feces and sera of patients in an outbreak of enterically transmitted non-A, non-E hepatitis, and this review covers the epidemiologic features and experimental infection of this novel virus.
Data sources come from our own work on this subject, published and unpublished.
Mainly our own work is included, and related literature is collected.
In an outbreak of enterically transmitted non-A-E hepatitis among students, a total of 381 cases (60.7%) were documented. Viral fragments identical to transfusion transmitted virus (TTV) were detected in both serum and stool samples. Asymptomatic virus carriers among the staff had positive serum (32.1%) and feces (24.6%), clearly a potential source of infection. This viral infection prevalence in 2 remote villages in northern and southern China was 9.2% and 10.6%, respectively, suggesting that China is an endemic area. In this study, groups of 3 Rhesus monkeys were infected via oral or intravenous inoculation with patient feces. Two additional monkeys were infected by passage. The virus was detected in serum, peripheral blood mononuclear cell (PBMC), liver, spleen and small intestine, while the virus positive single strand, which might be a replicative intermediate, was only in liver, intestine and PBMC of all animals.
This nonenveloped DNA virus might be transmitted both by blood and enteric routes. Considering its wide distribution and high prevalence, we suppose that nonparenteral transmission is more important.
检测经肠道传播的非甲非戊型肝炎暴发流行患者粪便和血清中的病毒，并综述这种新型病毒的流行病学特征和实验感染情况。
资料来源为本课题已发表和未发表的研究成果。
主要纳入本课题研究成果，并收集相关文献。
在一次学生经肠道传播的非甲非戊型肝炎暴发中，共记录到381例（60.7%）。在血清和粪便样本中均检测到与输血传播病毒（TTV）相同的病毒片段。工作人员中的无症状病毒携带者血清阳性率为32.1%，粪便阳性率为24.6%，显然是潜在的传染源。在中国北方和南方的2个偏远村庄，这种病毒感染率分别为9.2%和10.6%，提示中国是流行区。在本研究中，将3组恒河猴经口或静脉接种患者粪便进行感染。另外2只猴子通过传代感染。在血清、外周血单个核细胞（PBMC）、肝脏、脾脏和小肠中检测到病毒，而病毒阳性单链（可能是复制中间体）仅在所有动物的肝脏、肠道和PBMC中检测到。
这种无包膜DNA病毒可能通过血液和肠道途径传播。考虑到其广泛分布和高感染率，我们认为非肠道外传播更为重要。
